TY  - JOUR
A1  - Shiyan, Anna
A1  - Thompson, Melanie
A1  - Köcher, Saskia
A1  - Tausendschön, Michaela
A1  - Santos, Helena
A1  - Hänelt, Inga
A1  - Müller, Volker
T1  - Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus
T2  - Frontiers in microbiology
N2  - Halobacillus halophilus, a moderately halophilic bacterium isolated from salt marshes, produces various compatible solutes to cope with osmotic stress. Glutamate and glutamine are dominant compatible solutes at mild salinities. Glutamine synthetase activity in cell suspensions of Halobacillus halophilus wild type was shown to be salt dependent and chloride modulated. A possible candidate to catalyze glutamine synthesis is glutamine synthetase A2, whose transcription is stimulated by chloride. To address the role of GlnA2 in the biosynthesis of the osmolytes glutamate and glutamine, a deletion mutant (ΔglnA2) was generated and characterized in detail. We compared the pool of compatible solutes and performed transcriptional analyses of the principal genes controlling the solute production in the wild type strain and the deletion mutant. These measurements did not confirm the hypothesized role of GlnA2 in the osmolyte production. Most likely the presence of another, yet to be identified enzyme has the main contribution in the measured activity in crude extracts and probably determines the total chloride-modulated profile. The role of GlnA2 remains to be elucidated.
KW  - Halobacillus halophilus
KW  - glutamine synthetase
KW  - compatible solutes
KW  - osmoregulation
KW  - halophile
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52565
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-525650
SN  - 1664-302X
N1  - Copyright © 2014 Shiyan, Thompson, Köcher, Tausendschön, Santos, Hänelt and Müller. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 5
IS  - Art. 168
SP  - 1
EP  - 11
PB  - Frontiers Media
CY  - Lausanne
ER  -