TY - JOUR A1 - Vendl, Oliver A1 - Wawrosch, Christoph A1 - Noe, Christian R. A1 - Molina Medina, Carlos Mauricio A1 - Kahl, Günter A1 - Kopp, Brigitte T1 - Diosgenin contents and DNA fingerprint screening of various Yam (Dioscorea sp.) genotypes T2 - Zeitschrift für Naturforschung, C N2 - In addition to the importance of many Dioscorea species (yams) as starchy staple food, some representatives are known and still used as a source for the steroidal sapogenin diosgenin, which, besides phytosterols derived from tall-oil, is an important precursor for partial synthesis of steroids for pharmaceutical research and applications. While in edible yams the diosgenin content should be as low as possible, a high yield of the compound is preferable for cultivars which are grown for the extraction of sterols. In the past, miscalculations and insufficiently precise techniques for quantification of diosgenin prevailed. Therefore we set out to re-evaluate the steroid content of a world collection of Dioscorea species, using leaves as sample material. We optimized diosgenin quantification techniques and fingerprinted the whole collection with the DNA amplification fingerprinting (DAF) technique. Total diosgenin contents ranged from 0.04 to 0.93% of dry weight within the collection. Several Dioscorea cultivars can be characterized via their DAF fingerprint patterns. KW - Dioscorea KW - Diosgenin Production KW - DNA Amplification Fingerprinting Y1 - 2014 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79990 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-799902 SN - 0939-5075 SN - 1865-7125 VL - 61.2006 IS - 11-12 SP - 847 EP - 855 PB - Verlag der Zeitschrift für Naturforschung CY - Tübingen ER -