TY  - JOUR
A1  - Dold, Annabelle Cynthia Elisabeth
A1  - Han, Hong
A1  - Liu, Niankun
A1  - Hildebrandt, Andrea
A1  - Brüggemann, Mirko
A1  - Rücklé, Cornelia
A1  - Hänel, Heike
A1  - Busch, Anke
A1  - Beli, Petra
A1  - Zarnack, Katharina
A1  - König, Julian
A1  - Roignant, Jean-Yves
A1  - Lasko, Paul
T1  - Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation
T2  - PLoS Genetics
N2  - Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila, maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar (osk). We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3’ UTR in a region adjacent to A-rich sequences. Using Drosophila S2R+ cultured cells we show that this binding site overlaps with a Bruno1 (Bru1) responsive element (BREs) that regulates osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3’ UTR.
KW  - Oocytes
KW  - Messenger RNA
KW  - Ovaries
KW  - 3' UTR
KW  - Embryos
KW  - Drosophila melanogaster
KW  - Protein translation
KW  - Sequence motif analysis
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52553
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-525537
SN  - 1553-7404
SN  - 1553-7390
N1  - Copyright: © 2020 Dold et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 16
IS  - (1): e1008581
SP  - 1
EP  - 31
PB  - Public Library of Science
CY  - San Francisco, Calif.
ER  -