TY  - JOUR
A1  - Johnscher, Gerd
A1  - Berghäuser, Jürgen
A1  - Woenckhaus, Christoph
T1  - Darstellung und Eigenschaften von 5-Acetyl-4-methyl-1-(β -D-ribofuranosyl)-imidazol-5′-di- und -triphosphat
T1  - Synthesis and properties of 5-acetyl-4-methyl-1-(β-D-ribofuranosyl)-imidazole-5′ di- and -triphosphate
T2  - Zeitschrift für Naturforschung, C
N2  - 5-Acetyl-4-methyl-1-(β-D-ribofuranosyl) -imidazole-5′-phosphate reacts with diphenylphospho chloridate forming the asymmetrical pyrophosphate ester. This in turn reacts with tri-n-butylammonium phosphate yielding 5-acetyl-4-methyl-imidazole-riboside-5′-diphosphate and with tri-rcbutylammonium pyrophosphate to give the nucleotide triphosphate.
5-Acetyl-4-methyl-imidazole-riboside-5′-pyrophosphate shows in the test with pyruvate kinase a reaction rate three times slower than that of ADP; but the same Km as that of ADP. The ATP analogue is only about 10% as effective as ATP itself in the test with hexokinase, 3-phosphoglycerate kinase and gluconate kinase. Adenylate kinase and NAD+ kinase show no activity when ATP is replaced by the nucleotide-triphosphate-analogue. In presence of ATP the analogue strongly inhibits the reaction of adenylate kinase.
KW  - Nucleotide Triphosphate Analogue
KW  - Kinases
KW  - Enzymatic Behaviour
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72227
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-722278
SN  - 0939-5075
SN  - 1865-7125
VL  - 30.1975
IS  - 1-2
SP  - 25
EP  - 28
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -