TY - JOUR A1 - Lodemann, Edgar A1 - Wacker, Adolf T1 - Enzymatische Konfigurationsbestimmung von Nucleosiden T2 - Zeitschrift für Naturforschung, B N2 - It is possible to determine the anomeric configuration of nucleosides by a simple, spectrophotometric assay, using the nucleoside phosphorylase activity of cell-free extracts from E. coli. β-nucleo-sides are split, a-anomers remain unchanged. For a single estimation 20-40 µg of nucleoside are required. 6-Azauracil- and 8-azaguanine-β-ᴅ-riboside and some nucleoside phosphates are resistant, a fact, which is of interest in view of the specificity of nucleoside phosphorylases. Y1 - 2014 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74869 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-748692 SN - 1865-7117 SN - 0932-0776 VL - 22 IS - 1 SP - 42 EP - 44 PB - Verlag der Zeitschrift für Naturforschung CY - Tübingen ER -