TY  - JOUR
A1  - Narin Sontigun, 
A1  - Kabkaew L. Sukontason, 
A1  - Amendt, Jens
A1  - Zajac, Barbara Karolina
A1  - Zehner, Richard
A1  - Kom Sukontason, 
A1  - Theeraphap Chareonviriyaphap, 
A1  - Anchalee Wannasan, 
T1  - Molecular analysis of forensically important blow flies in Thailand
T2  - Insects
N2  - Blow flies are the first insect group to colonize on a dead body and thus correct species identification is a crucial step in forensic investigations for estimating the minimum postmortem interval, as developmental times are species-specific. Due to the difficulty of traditional morphology-based identification such as the morphological similarity of closely related species and uncovered taxonomic keys for all developmental stages, DNA-based identification has been increasing in interest, especially in high biodiversity areas such as Thailand. In this study, the effectiveness of long mitochondrial cytochrome c oxidase subunit I and II (COI and COII) sequences (1247 and 635 bp, respectively) in identifying 16 species of forensically relevant blow flies in Thailand (Chrysomya bezziana, Chrysomya chani, Chrysomya megacephala, Chrysomya nigripes, Chrysomya pinguis, Chrysomya rufifacies, Chrysomya thanomthini, Chrysomya villeneuvi, Lucilia cuprina, Lucilia papuensis, Lucilia porphyrina, Lucilia sinensis, Hemipyrellia ligurriens, Hemipyrellia pulchra, Hypopygiopsis infumata, and Hypopygiopsis tumrasvini) was assessed using distance-based (Kimura two-parameter distances based on Best Match, Best Close Match, and All Species Barcodes criteria) and tree-based (grouping taxa by sequence similarity in the neighbor-joining tree) methods. Analyses of the obtained sequence data demonstrated that COI and COII genes were effective markers for accurate species identification of the Thai blow flies. This study has not only demonstrated the genetic diversity of Thai blow flies, but also provided a reliable DNA reference database for further use in forensic entomology within the country and other regions where these species exist.
KW  - forensic entomology
KW  - molecular identification
KW  - COI
KW  - COII
KW  - blow flies
KW  - Thailand
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55031
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-550317
SN  - 2075-4450
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 9.2018
IS  - 4, Art. 159
SP  - 1
EP  - 15
PB  - MDPI
CY  - Basel
ER  -