TY  - JOUR
A1  - Wiechmann, Anja
A1  - Müller, Volker
T1  - Energy conservation in the acetogenic bacterium clostridium aceticum
T2  - Microorganisms
N2  - In times of global warming caused by the extensive use of fossil fuels, the need to capture gaseous carbon compounds is growing bigger. Several groups of microorganisms can fix the greenhouse gas CO2. Out of these, acetogenic bacteria are role models in their ability to reduce CO2 with hydrogen to acetate, which makes acetogens prime candidates for genetic modification towards biotechnological production of value-added compounds from CO2, such as biofuels. However, growth of acetogens on gaseous substrates is strongly energy-limited, and successful metabolic engineering requires a detailed knowledge of the bioenergetics. In 1939, Clostridium aceticum was the first acetogen to be described. A recent genomic study revealed that this organism contains cytochromes and therefore may use a proton gradient in its respiratory chain. We have followed up these studies and will present data that C. aceticum does not use a H+ but a Na+ gradient for ATP synthesis, established by a Na+-Rnf. Experimental data and in silico analyses enabled us to propose the biochemistry and bioenergetics of acetogenesis from H2 + CO2 in C. aceticum.
KW  - energy conservation
KW  - respiratory chain
KW  - acetogenic bacteria
KW  - Wood–Ljungdahl pathway
KW  - ATP synthase
KW  - Rnf complex
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57177
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-571777
SN  - 2076-2607
VL  - 9
IS  - Article 258
PB  - MDPI
CY  - Basel
ER  -