TY  - JOUR
A1  - Valečka, Jan
A1  - Camosseto, Voahirana
A1  - McEwan, David G.
A1  - Liu, Zhuangzhuang
A1  - Strock, Eva
A1  - Almeida, Catarina R.
A1  - Su, Bing
A1  - Đikić, Ivan
A1  - Liang, Yinming
A1  - Gatti, Evelina
A1  - Pierre, Philippe
T1  - RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
T2  - Royal Society Open Science
N2  - We report here that RUFY4, a newly characterized member of the ‘RUN and FYVE domain-containing’ family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
KW  - RUFY
KW  - mitophagy
KW  - Skp
KW  - alveolar macrophages
KW  - LPS
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62916
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-629166
SN  - 2054-5703
N1  - The project and J.V. were supported by grants from l'Agence Nationale de la Recherche (ANR) ‘DCBIOL Labex ANR-11-LABEX-0043’, ‘INFORM Labex ANR-11-LABEX-0054’ funded by the ‘Investissements d'Avenir’ French government programme. This work was also financially supported by the project PTDC/BIA-CEL/28791/2017 and POCI-01-0145-FEDER-028791, as well as POCI-01-0145-FEDER-030882 and PTDC/BIA-MOL/30882/2017, funded by FEDER, through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES.
N1  - Data accessibility
The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD026728.
N1  - Electronic supplementary material is available online at https://doi.org/10.6084/m9.figshare.c.5509720.
VL  - 8
IS  - 7
SP  - 1
EP  - 15
PB  - Royal Soc. Publ.
CY  - London
ER  -