TY  - JOUR
A1  - Kwankamol Limsopatham, 
A1  - Hall, Martin J. R.
A1  - Zehner, Richard
A1  - Zajac, Barbara Karolina
A1  - Verhoff, Marcel A.
A1  - Narin Sontigun, 
A1  - Kom Sukontason, 
A1  - Kabkaew L. Sukontason, 
A1  - Amendt, Jens
T1  - A molecular, morphological, and physiological comparison of English and German populations of Calliphora vicina (Diptera: Calliphoridae)
T2  - PLoS one
N2  - The bluebottle blow fly Calliphora vicina is a common species distributed throughout Europe that can play an important role as forensic evidence in crime investigations. Developmental rates of C. vicina from distinct populations from Germany and England were compared under different temperature regimes to explore the use of growth data from different geographical regions for local case work. Wing morphometrics and molecular analysis between these populations were also studied as indicators for biological differences. One colony each of German and English C. vicina were cultured at the Institute of Legal Medicine in Frankfurt, Germany. Three different temperature regimes were applied, two constant (16°C & 25°C) and one variable (17–26°C, room temperature = RT). At seven time points (600, 850, 1200, 1450, 1800, 2050, and 2400 accumulated degree hours), larval lengths were measured; additionally, the durations of the post feeding stage and intrapuparial metamorphosis were recorded. For the morphometric and molecular study, 184 females and 133 males from each C. vicina population (Germany n = 3, England n = 4) were sampled. Right wings were measured based on 19 landmarks and analyzed using canonical variates analysis and discriminant function analysis. DNA was isolated from three legs per specimen (n = 61) using 5% chelex. A 784 bp long fragment of the mitochondrial cytochrome b gene was sequenced; sequences were aligned and phylogenetically analyzed. Similar larval growth rates of C. vicina were found from different geographic populations at different temperatures during the major part of development. Nevertheless, because minor differences were found a wider range of temperatures and sampling more time points should be analyzed to obtain more information relevant for forensic case work. Wing shape variation showed a difference between the German and English populations (P<0.0001). However, separation between the seven German and English populations at the smaller geographic scale remained ambiguous. Molecular phylogenetic analysis by maximum likelihood method could not unambiguously separate the different geographic populations at a national (Germany vs England) or local level.
KW  - Morphometry
KW  - Germany
KW  - German people
KW  - England
KW  - Animal wings
KW  - Phylogenetic analysis
KW  - Forensics
KW  - Sequence alignment
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48425
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-484259
SN  - 1932-6203
N1  - Copyright: © 2018 Limsopatham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 13.2018
IS  - (12): e0207188
SP  - 1
EP  - 22
PB  - PLoS
CY  - Lawrence, Kan.
ER  -