TY  - JOUR
A1  - Yap, Kenrick
A1  - Drakew, Alexander
A1  - Smilovic, Dinko
A1  - Rietsche, Michael
A1  - Paul, Mandy H.
A1  - Vuksic, Mario
A1  - Del Turco, Domenico
A1  - Deller, Thomas
T1  - The actin-modulating protein synaptopodin mediates long-term survival of dendritic spines
T2  - eLife
N2  - Large spines are stable and important for memory trace formation. The majority of large spines also contains synaptopodin (SP), an actin-modulating and plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence of SP within large spines could explain their long lifetime. Indeed, using 2-photon time-lapse imaging of SP-transgenic granule cells in mouse organotypic tissue cultures we found that spines containing SP survived considerably longer than spines of equal size without SP. Of note, SP-positive (SP+) spines that underwent pruning first lost SP before disappearing. Whereas the survival time courses of SP+ spines followed conditional two-stage decay functions, SP-negative (SP-) spines and all spines of SP-deficient animals showed single-phase exponential decays. This was also the case following afferent denervation. These results implicate SP as a major regulator of long-term spine stability: SP clusters stabilize spines, and the presence of SP indicates spines of high stability.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62367
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-623671
SN  - 2050-084X
VL  - 9
IS  - art. e62944
SP  - 1
EP  - 31
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -