TY  - JOUR
A1  - Koenig, Friederike
T1  - Anacystis mutants with different tolerances to DCMU-type herbicides show differences in architecture and dynamics of the photosynthetic apparatus, depending on site and mode of the amino acid exchanges in the D1 protein
T2  - Zeitschrift für Naturforschung, C
N2  - Mutants of Anacystis R2 with different amino acid exchanges in positions 255 and/or 264 in copy I of the psbA gene, leading to different tolerances to DCMU-type herbicides, are com- pared with the respective wild type concerning pigmentation and incorporation of 35S into the D1 protein upon growth in the presence of [35S]methionine. All mutants have shade-type appearance compared to the wild type, although to different extents depending on site and mode of the amino acid exchange in the D1 protein. Except for 3 mutants, there is no correlation between shade-type appearance on one hand and resistance towards a certain inhibitor on the other hand.
Not only the molar ratio of phycocyanin (PC) to chlorophyll (Chi) is higher in all mutants compared to the respective wild type, but also the rate of synthesis of the D1 protein. On the background of different levels of total 35S incorporation within 18 min, D1 synthesis can be related to shade adaptation. Degradation of the D1 protein remains to be thoroughly studied in this context.
No reproducible differences in whole chain electron transport were observed between mutants and wild type.
KW  - Cyanobacteria
KW  - Herbicide-Tolerant Mutants
KW  - DCMU-Type Inhibitors
KW  - Regulation of D1 Protein
KW  - Shade Adaptation
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79911
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-799118
SN  - 0939-5075
SN  - 1865-7125
VL  - 45.1990
IS  - 5
SP  - 446
EP  - 451
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -