TY  - JOUR
A1  - Haigis, Ann-Cathrin
A1  - Ottermanns, Richard
A1  - Schiwy, Andreas Herbert
A1  - Hollert, Henner
A1  - Legradi, Jessica
T1  - Getting more out of the zebrafish light dark transition test
T2  - Chemosphere
N2  - In (eco-)toxicological studies the light/dark transition (LDT) test is one of the most frequently used behaviour assays with zebrafish eleutheroembryos. However, study results vary regarding data presentation and analysis and mostly focus on a limited amount of the recorded data. In this study, we investigated whether monitoring two behavioural outcomes (time and distance moved) together with analysing multiple parameters can improve test sensitivity and data interpretation. As a proof of principle 5-day old zebrafish (Danio rerio) eleutheroembryos exposed to either endocrine disruptors (EDs) or acetylcholine esterase (AChE) inhibitors were investigated. We analysed conventional parameters such as mean and sum and implemented additional endpoints such as minimum or maximum distance moved and new parameters assessing the bursting response of eleutheroembryos. Furthermore, changes in eleutheroembryonic behaviour during the moment of the light to dark transition were added. To improve data presentation control-normalised results were displayed in radar charts, enabling the simultaneous presentation of different parameters in relation to each other. This enabled us to identify parameters most relevant to a certain behavioural response. A cut off threshold using control data was applied to identify parameters that were altered in a biological relevant manner. Our approach was able to detect effects on different parameters that remained undetected when analysis was done using conventional bar graphs on - in most cases analysed - averaged, mean distance moved values. By combining the radar charts with additional parameters and by using control-based thresholds, we were able to increase the test sensitivity and promote a deeper understanding of the behaviour response of zebrafish eleutheroembryos in the LDT test and thereby increased its usability for behavioural toxicity studies.
KW  - Behaviour
KW  - Endocrine disruptors
KW  - Light dark transition test
KW  - Organophosphates
KW  - Zebrafish eleutheroembryo
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78432
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-784320
SN  - 1879-1298
N1  - The authors thank the German federal ministry of education and science BMBF (Project number 02WRS1419C) for their support.
VL  - 295
IS  - art. 133863
SP  - 1
EP  - 11
PB  - Elsevier Science
CY  - Amsterdam [u.a.]
ER  -