TY  - JOUR
A1  - Maurer, Gabriele D.
A1  - Heller, Sonja
A1  - Wanka, Christina
A1  - Rieger, Johannes
A1  - Steinbach, Joachim Peter
T1  - Knockdown of the TP53-induced glycolysis and apoptosis regulator (TIGAR) sensitizes glioma cells to hypoxia, irradiation and temozolomide
T2  - International journal of molecular sciences
N2  - The TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to decrease glycolysis, to activate the pentose phosphate pathway, and to provide protection against oxidative damage. Hypoxic regions are considered characteristic of glioblastoma and linked with resistance to current treatment strategies. Here, we established that LNT-229 glioma cell lines stably expressed shRNA constructs targeting TIGAR, and exposed them to hypoxia, irradiation and temozolomide. The disruption of TIGAR enhanced levels of reactive oxygen species and cell death under hypoxic conditions, as well as the effectiveness of irradiation and temozolomide. In addition, TIGAR was upregulated by HIF-1α. As a component of a complex network, TIGAR contributes to the metabolic adjustments that arise from either spontaneous or therapy-induced changes in tumor microenvironment.
KW  - TP53-induced glycolysis and apoptosis regulator
KW  - glioma
KW  - hypoxia
KW  - irradiation
KW  - temozolomide
KW  - reactive oxygen species
KW  - hypoxia-inducible factor
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48475
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-484757
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
VL  - 20
IS  - 5, Art. 1061
SP  - 1
EP  - 14
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -