TY  - JOUR
A1  - Klapper, Simon
A1  - Sauter, Evelyn J.
A1  - Swiersy, Anka
A1  - Hyman, Max A. E.
A1  - Bamann, Christian
A1  - Bamberg, Ernst
A1  - Busskamp, Volker
T1  - On-demand optogenetic activation of human stem-cell-derived neurons
T2  - Scientific reports
N2  - The widespread application of human stem-cell-derived neurons for functional studies is impeded by complicated differentiation protocols, immaturity, and deficient optogene expression as stem cells frequently lose transgene expression over time. Here we report a simple but precise Cre-loxP-based strategy for generating conditional, and thereby stable, optogenetic human stem-cell lines. These cells can be easily and efficiently differentiated into functional neurons, and optogene expression can be triggered by administering Cre protein to the cultures. This conditional expression system may be applied to stem-cell-derived neurons whenever timed transgene expression could help to overcome silencing at the stem-cell level.
KW  - Induced pluripotent stem cells
KW  - Optogenetics
KW  - Stem-cell differentiation
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44997
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-449978
SN  - 2045-2322
N1  - Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017
VL  - 7
IS  - 1, Art. 14450
SP  - 1
EP  - 9
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - [London]
ER  -