TY  - JOUR
A1  - Hotz, Paul W.
A1  - Wiesnet, Marion
A1  - Tascher, Georg
A1  - Braun, Thomas
A1  - Müller, Stefan
A1  - Mendler, Luca
T1  - Profiling the murine SUMO proteome in response to cardiac ischemia and reperfusion injury
T2  - Molecules
N2  - SUMOylation is a reversible posttranslational modification pathway catalyzing the conjugation of small ubiquitin-related modifier (SUMO) proteins to lysine residues of distinct target proteins. SUMOylation modifies a wide variety of cellular regulators thereby affecting a multitude of key processes in a highly dynamic manner. The SUMOylation pathway displays a hallmark in cellular stress-adaption, such as heat or redox stress. It has been proposed that enhanced cellular SUMOylation protects the brain during ischemia, however, little is known about the specific regulation of the SUMO system and the potential target proteins during cardiac ischemia and reperfusion injury (I/R). By applying left anterior descending (LAD) coronary artery ligation and reperfusion in mice, we detect dynamic changes in the overall cellular SUMOylation pattern correlating with decreased SUMO deconjugase activity during I/R injury. Further, unbiased system-wide quantitative SUMO-proteomics identified a sub-group of SUMO targets exhibiting significant alterations in response to cardiac I/R. Notably, transcription factors that control hypoxia- and angiogenesis-related gene expression programs, exhibit altered SUMOylation during ischemic stress adaptation. Moreover, several components of the ubiquitin proteasome system undergo dynamic changes in SUMO conjugation during cardiac I/R suggesting an involvement of SUMO signaling in protein quality control and proteostasis in the ischemic heart. Altogether, our study reveals regulated candidate SUMO target proteins in the mouse heart, which might be important in coping with hypoxic/proteotoxic stress during cardiac I/R injury.
KW  - SENP
KW  - SUMO
KW  - cardiac I/R injury
KW  - immunoprecipitation
KW  - mass spectrometry
KW  - proteomics
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57551
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-575515
SN  - 1420-3049
VL  - 25
IS  - Article 5571
PB  - MDPI
CY  - Basel
ER  -