TY  - JOUR
A1  - Chowdhury, Nilanjan Pal
A1  - Litty, Dennis
A1  - Müller, Volker
T1  - Biosynthesis of butyrate from methanol and carbon monoxide by recombinant Acetobacterium woodii
T2  - International microbiology
N2  - Methanol is one of the most widely produced organic substrates from syngas and can serve as a bio-feedstock to cultivate acetogenic bacteria which allows a major contribution to reducing greenhouse gas. Acetobacterium woodii is one of the very few acetogens that can utilize methanol to produce acetate as sole product. Since A. woodii is genetically tractable, it is an interesting candidate to introduce recombinant pathways for production of bio-commodities from methanol. In this study, we introduced the butyrate production operon from a related acetogen, Eubacterium callanderi KIST612, into A. woodii and show a stable production of butyrate from methanol. This study also reveals how butyrate production by recombinant A. woodii strains can be enhanced with addition of electrons in the form of carbon monoxide. Our results not only show a stable expression system of non-native enzymes in A. woodii but also increase in the product spectrum of A. woodii to compounds with higher economic value.
KW  - Acetogen
KW  - Butyrate
KW  - Bioengineering
KW  - Wood-Ljungdahl pathway
KW  - Acetogenesis
KW  - Carbon cycling
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69572
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695729
SN  - 1618-1905
N1  - Open Access funding enabled and organized by Projekt DEAL. The research was funded by an Advanced Grant of the European Research Council under the European Union’s Horizon 2020 Research and Innovation Program (grant agreement no. 741791).
VL  - 25
IS  - 3
SP  - 551
EP  - 560
PB  - Spanish Soc. for Microbiology
CY  - Madrid
ER  -