TY  - JOUR
A1  - Dörrbaum, Aline Ricarda
A1  - Alvarez-Castelao, Beatriz
A1  - Nassim-Assir, Belquis
A1  - Langer, Julian David
A1  - Schuman, Erin M.
T1  - Proteome dynamics during homeostatic scaling in cultured neurons
T2  - eLife
N2  - Protein turnover, the net result of protein synthesis and degradation, enables cells to remodel their proteomes in response to internal and external cues. Previously, we analyzed protein turnover rates in cultured brain cells under basal neuronal activity and found that protein turnover is influenced by subcellular localization, protein function, complex association, cell type of origin, and by the cellular environment (Dörrbaum et al., 2018). Here, we advanced our experimental approach to quantify changes in protein synthesis and degradation, as well as the resulting changes in protein turnover or abundance in rat primary hippocampal cultures during homeostatic scaling. Our data demonstrate that a large fraction of the neuronal proteome shows changes in protein synthesis and/or degradation during homeostatic up- and down-scaling. More than half of the quantified synaptic proteins were regulated, including pre- as well as postsynaptic proteins with diverse molecular functions.
KW  - protein synthesis
KW  - protein degradation
KW  - synaptic scaling
KW  - protein turnover
KW  - homeostasis
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/85725
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-857258
SN  - 2050-084X
VL  - 9
IS  - e52939
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -