TY  - JOUR
A1  - Brendel, Martin
A1  - Fäth, Wolfgang Walter
T1  - Isolation and characterization of mutants of saccharomyces cerevisiae auxotrophic and conditionally auxotrophic for 5′-dTMP
T2  - Zeitschrift für Naturforschung, C
N2  - An improved method for isolation of yeast m utants auxotrophic for 5′-dTM P is presented. The procedure employs the two folic acid antagonists am inopterin and sulfanilam ide (SAA). Selectiveness of the procedure depends on concentration of SAA and time of incubation.
44 mutants auxotrophic and 3 conditionally auxotrophic for 5′-dTMP were isolated. All belong to one complementation group. The corresponding gene was designated TMP1. Tetrad dissection revealed its chromosomal nature. TMP1 is not closely linked to the genes ADE2,, LEU1, ARG 4, ILV2, HIS5, LYS1 and the mating type locus. With the centromere-linked genes ARG4 and LEU1 I gene TMP1 exhibited second division segregation frequencies of 0.42 and 0.53 respectively, indicative of centromere-linkage.
Strains auxotrophic and conditionally auxotrophic for 5′-dTM P were all respiratory deficient (petite). Genetical analysis indicates that the petite phenotype is due to loss of the rho factor in cells harbouring either tmp1 or tmp1ts alleles.
KW  - Folic Acid Antagonists
KW  - Thymidylate Synthetase
KW  - Tetrad Analysis
KW  - Elimination of Rho Factor
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/81183
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-811837
SN  - 0939-5075
SN  - 1865-7125
VL  - 29.1974
IS  - 11-12
SP  - 733
EP  - 738
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -