TY  - JOUR
A1  - Bergs, Amelie
A1  - Liewald, Jana F.
A1  - Rodriguez-Rozada, Silvia
A1  - Liu, Qiang
A1  - Wirt, Christin
A1  - Bessel, Artur
A1  - Zeitzschel, Nadja
A1  - Durmaz, Hilal
A1  - Nozownik, Adrianna
A1  - Dill, Holger
A1  - Jospin, Maëlle
A1  - Vierock, Johannes Tobias Theodor
A1  - Bargmann, Cornelia Isabella
A1  - Hegemann, Peter
A1  - Wiegert, Simon
A1  - Gottschalk, Alexander
T1  - All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals
T2  - Nature Communications
N2  - Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74245
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-742452
SN  - 2041-1723
VL  - 14
IS  - Article number
PB  - Nature Publishing Group
CY  - London
ER  -