TY  - JOUR
A1  - Lutsak, Tetiana
A1  - Fernández Mendoza, Fernando
A1  - Tzovaras, Bastian Greshake
A1  - Dal Grande, Francesco
A1  - Ebersberger, Ingo
A1  - Ott, Sieglinde
A1  - Printzen, Christian
T1  - Characterization of microsatellite loci in the lichen-forming fungus cetraria aculeata (parmeliaceae, ascomycota)
T2  - Applications in plant sciences
N2  - Premise of the study: Polymorphic microsatellite markers were developed for the lichen species Cetraria aculeata (Parmeliaceae) to study fine-scale population diversity and phylogeographic structure.
Methods and Results: Using Illumina HiSeq and MiSeq, 15 fungus-specific microsatellite markers were developed and tested on 81 specimens from four populations from Spain. The number of alleles ranged from four to 13 alleles per locus with a mean of 7.9, and average gene diversities varied from 0.40 to 0.73 over four populations. The amplification rates of 10 markers (CA01– CA10) in populations of C. aculeata exceeded 85%. The markers also amplified across a range of closely related species, except for locus CA05, which did not amplify in C. australiensis and C. "panamericana," and locus CA10 which did not amplify in C. australiensis.
Conclusions: The identified microsatellite markers will be used to study the genetic diversity and phylogeographic structure in populations of C. aculeata in western Eurasia.
KW  - Cetraria aculeata
KW  - lichen
KW  - microsatellites
KW  - Parmeliaceae
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42265
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-422651
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033364
SN  - 2168-0450
N1  - © 2016 Lutsak et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
VL  - 4
IS  - (9):1600047
SP  - 1
EP  - 4
PB  - BioOne
CY  - Washington, DC
ER  -