TY  - JOUR
A1  - Gehatia, Matatiahu
T1  - Bestimmung des Molekulargewichtes der 20-β-Hydroxy-steroid-dehydrogenase
T2  - Zeitschrift für Naturforschung, B
N2  - The enzyme 20-β-Hydroxy-steroid-dehydrogenase obtained from the culture of Streptomyces hydrogenans and dissolved in 0.05 M Tris puffer, pH 7.3, has been investigated by means of a ultracentrifuge at 20 °C. The sedimentation- as well as the diffusion-coefficients obtained from various solutions at different concentrations were extrapolated to the concentration c = 0. The resulting zero-value for the sedimentation coefficient is s0 = 6.64 s and for the diffusion coefficient is D0 = 5.51 × 10-7 cm2/sec. Supposing the partial specific volume of the enzyme under consideration analogously to other similar proteins is V+=0.749 ml/g, the molecular weight has been estimated as M = 118 400.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69890
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-698903
SN  - 1865-7117
SN  - 0932-0776
VL  - 17
IS  - 7
SP  - 432
EP  - 436
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -