TY  - JOUR
A1  - Schürmanns, Lea
A1  - Hamann, Andrea
A1  - Osiewacz, Heinz D.
T1  - Lifespan increase of podospora anserina by oleic acid is linked to alterations in energy metabolism, membrane trafficking and autophagy
T2  - Cells
N2  - The maintenance of cellular homeostasis over time is essential to avoid the degeneration of biological systems leading to aging and disease. Several interconnected pathways are active in this kind of quality control. One of them is autophagy, the vacuolar degradation of cellular components. The absence of the sorting nexin PaATG24 (SNX4 in other organisms) has been demonstrated to result in impairments in different types of autophagy and lead to a shortened lifespan. In addition, the growth rate and the size of vacuoles are strongly reduced. Here, we report how an oleic acid diet leads to longevity of the wild type and a PaAtg24 deletion mutant (ΔPaAtg24). The lifespan extension is linked to altered membrane trafficking, which abrogates the observed autophagy defects in ΔPaAtg24 by restoring vacuole size and the proper localization of SNARE protein PaSNC1. In addition, an oleic acid diet leads to an altered use of the mitochondrial respiratory chain: complex I and II are bypassed, leading to reduced reactive oxygen species (ROS) production. Overall, our study uncovers multiple effects of an oleic acid diet, which extends the lifespan of P. anserina and provides perspectives to explain the positive nutritional effects on human aging.
KW  - Podospora anserina
KW  - aging
KW  - autophagy
KW  - membrane trafficking
KW  - ATG24
KW  - peroxisomes
KW  - ER
KW  - mitochondria
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69295
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-692958
SN  - 2073-4409
N1  - This research was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project-ID 25913077–SFB1177 to H.D.O.
VL  - 11
IS  - 3, art. 519
SP  - 1
EP  - 23
PB  - MDPI
CY  - Basel
ER  -