TY  - INPR
A1  - Mohamed, Sara
A1  - Soppa, Jörg
T1  - The non-structural proteins NS3 and NS5A of Hepatitis C Virus (HCV) are degraded by two host proteolysis systems
T2  - bioRxiv
N2  - Each lifecycle of the Hepatitis C virus (HCV) produces structural and non-structural (NS) proteins in equimolar. Structural proteins were either assembled or degraded by host proteolysis systems, while NS proteins remain inside the host cells and don’t accumulate. Therefore, they must be degraded. Here, NS3 and NS5A half-lives were quantified in the presence of autolysosome and proteasome different modulators. Inhibitors of both systems increased the half-life, while inducers decreased the half-life. Furthermore, polyubiquitination of NS3 and NS5A was observed. Additionally, their intracellular co-localization with autolysosome (LAMP2) and proteasome (PSMB5) was observed, and inhibitors of both systems increased the degree of co-localization. A better understanding of NS protein degradation might help to improve medical interventions during HCV infections in the future.
KW  - HCV
KW  - non-structural proteins
KW  - NS3
KW  - NS5A
KW  - protein degradation
KW  - proteolysis systems
KW  - autophagy
KW  - proteasome
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75418
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-754186
UR  - https://www.biorxiv.org/content/10.1101/2023.08.16.553615v2
IS  - 2023.08.16.553615 Version 2
ER  -