TY  - JOUR
A1  - Wagner, Jacqueline
A1  - Schäfer, Dominik
A1  - Eichen, Nikolas von den
A1  - Haimerl, Christina
A1  - Harth, Simon
A1  - Oreb, Igor-Mislav
A1  - Benz, Philipp
A1  - Weuster-Botz, Dirk
T1  - D-Galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate
T2  - Applied microbiology and biotechnology
N2  - Pectin-rich residues are considered as promising feedstocks for sustainable production of platform chemicals. Enzymatic hydrolysis of extracted sugar beet press pulp (SBPP) releases the main constituent of pectin, d-galacturonic acid (d-GalA). Using engineered Saccharomyces cerevisiae, d-GalA is then reduced to l-galactonate (l-GalOA) with sorbitol as co-substrate. The current work addresses the combination of enzymatic hydrolysis of pectin in SBPP with a consecutive optimized biotransformation of the released d-GalA to l-GalOA in simple batch processes in stirred-tank bioreactors. Process conditions were first identified with synthetic media, where a product concentration of 9.9 g L-1 L-GalOA was obtained with a product selectivity of 99% (L-GalOA D-GalA-1) at pH 5 with 4% (w/v) sorbitol within 48 h. A very similar batch process performance with a product selectivity of 97% was achieved with potassium citrate buffered SBPP hydrolysate, demonstrating for the first time direct production of L-GalOA from hydrolyzed biomass using engineered S. cerevisiae. Combining the hydrolysis process of extracted SBPP and the biotransformation process with engineered S. cerevisiae paves the way towards repurposing pectin-rich residues as substrates for value-added chemicals.
KW  - Extracted sugar beet press pulp
KW  - Pectin
KW  - Enzymatic hydrolysis
KW  - D-Galacturonicacid
KW  - L-Galactonate
KW  - Saccharomyces cerevisiae
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69514
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695145
SN  - 1432-0614
N1  - Open access funding enabled and organized by Projekt DEAL. This work was supported by the German Federal Ministry of Education and Research [grant number 031B0342A-C].
VL  - 105
IS  - 14-15
SP  - 5795
EP  - 5807
PB  - Springer
CY  - Berlin ; Heidelberg ; New York
ER  -