TY  - JOUR
A1  - Lorenzen, Wolfram
A1  - Bozhüyük, Kenan A. J.
A1  - Cortina, Niña S.
A1  - Bode, Helge Björn
T1  - A comprehensive insight into the lipid composition of Myxococcus xanthus by UPLC-ESI-MS
T2  - Journal of lipid research
N2  - Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.
KW  - lipidomics
KW  - myxobacteria
KW  - ceramide phosphoinositols
KW  - ether lipids
KW  - lipid profiles
KW  - electrospray ionization-mass spectrometry
KW  - reversed phase
KW  - ultra-performance liquid chromatography
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76974
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-769746
SN  - 0022-2275
VL  - 55.2014
IS  - 12
SP  - 2620
EP  - 2633
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -