TY  - INPR
A1  - Acera Mateos, Pablo
A1  - Sethi, Aditya J.
A1  - Ravindran, Agin
A1  - Srivastava, Akanksha
A1  - Woodward, Katrina
A1  - Mahmud, Shafi
A1  - Kanchi, Madhu
A1  - Guarnacci, Marco
A1  - Xu, Jiajia
A1  - Yuen, Zaka Wing Sze
A1  - Zhou, You
A1  - Sneddon, Alexandra
A1  - Hamilton, William
A1  - Gao, Jing
A1  - Starrs, Lora M.
A1  - Hayashi, Rippei
A1  - Wickramasinghe, Vihandha
A1  - Zarnack, Katharina
A1  - Preiss, Thomas
A1  - Burgio, Gaetan
A1  - Dehorter, Nathalie
A1  - Shirokikh, Nikolay
A1  - Eyras Jiménez, Eduardo Angel
T1  - Prediction of m6A and m5C at single-molecule resolution reveals a cooccurrence of RNA modifications across the transcriptome
T2  - bioRxiv
N2  - The epitranscriptome embodies many new and largely unexplored functions of RNA. A significant roadblock hindering progress in epitranscriptomics is the identification of more than one modification in individual transcript molecules. We address this with CHEUI (CH3 (methylation) Estimation Using Ionic current). CHEUI predicts N6-methyladenosine (m6A) and 5-methylcytidine (m5C) in individual molecules from the same sample, the stoichiometry at transcript reference sites, and differential methylation between any two conditions. CHEUI processes observed and expected nanopore direct RNA sequencing signals to achieve high single-molecule, transcript-site, and stoichiometry accuracies in multiple tests using synthetic RNA standards and cell line data. CHEUI’s capability to identify two modification types in the same sample reveals a co-occurrence of m6A and m5C in individual mRNAs in cell line and tissue transcriptomes. CHEUI provides new avenues to discover and study the function of the epitranscriptome.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83417
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-834174
UR  - https://www.biorxiv.org/content/10.1101/2022.03.14.484124v8
IS  - 2022.03.14.484124 Version 8
PB  - bioRxiv
ER  -