TY  - INPR
A1  - Haydo, Alicia
A1  - Wehle, Andrej
A1  - Herold-Mende, Christel
A1  - Kögel, Donat
A1  - Pampaloni, Francesco
A1  - Linder, Benedikt
T1  - Combining organotypic tissue culture with multi-color fluorescence light-sheet microscopy (OTCxLSFM) a novel tool to study glioma invasion/migration
T2  - bioRxiv
N2  - Glioblastoma is a very aggressive tumor and represents the most common primary brain malignancy. Key characteristics include its high resistance against conventional treatments, such as radio- and chemotherapy and its diffuse tissue infiltration, preventing complete surgical resection. The analysis of migration and invasion processes in a physiological microenvironment allows for enhanced understanding of these processes and can lead to improved therapeutic approaches. Here, we combine two state-of-the-art techniques, adult organotypic brain tissue slice culture (OTC) and light sheet fluorescence microscopy (LSFM) of cleared tissues in a combined method termed OTCxLSFM. Using this methodology, we can show that glioblastoma tissue infiltration can be effectively blocked through treatment with arsenic trioxide, as well as genetic depletion of the tetraspanin, transmembrane receptor CD9. With our analysis-pipeline we gain single-cell level, three-dimensional information, as well as insights into the morphological appearance of the tumor cells.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/73169
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-731691
IS  - 2023.02.09.527810
ER  -