TY  - JOUR
A1  - Gerlich, Wolfram H.
T1  - Größenbestimmung biologischer Partikel durch Gelchromatographie an Sepharose 6B
T1  - Determination of particle size by gel chromatography with Sepharose 6B
T2  - Zeitschrift für Naturforschung, B
N2  - Gel chromatography with 6% agarose gel (Sepharose 6B) can be used for measuring the Stokes’ radius of biological particles within the range of 1,5 nm and 35 nm. The molecular weight determination of proteins is not very reliable with this method. Hepatitis sera have been chromatographied to measure the size of hepatitis associated antigen (Australia-antigen).
The radius of this antigen was determined to be 10,3 nm, which agrees with the results of electron microscopy and ultracentrifugation. The Stokes’ radius of human serum IgM was found to be 10,6 nm.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69861
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-698610
SN  - 1865-7117
SN  - 0932-0776
VL  - 26
IS  - 10
SP  - 1040
EP  - 1044
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -