TY  - JOUR
A1  - Matsumura, Hideo
A1  - Yoshida, Kentaro
A1  - Luo, Shujun
A1  - Kimura, Eiji
A1  - Fujibe, Takahiro
A1  - Albertyn, Zayed
A1  - Barrero, Roberto A.
A1  - Krüger, Detlev H.
A1  - Kahl, Günter
A1  - Schroth, Gary P.
A1  - Terauchi, Ryohei
T1  - High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing
T2  - PLoS One
N2  - We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries. Here, we demonstrated that HT-SuperSAGE provided highly sensitive, reproducible and accurate digital gene expression data. By increasing throughput for analysis in HT-SuperSAGE, various applications are foreseen and several examples are provided in the present study, including analyses of laser-microdissected cells, biological replicates and tag extraction using different anchoring enzymes.
Y1  - 2010
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/22621
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-114660
N1  - Copyright: © 2010 Matsumura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 5
IS  - (8): e12010
ER  -