TY  - JOUR
A1  - Schmidt, Martin
A1  - Cömer, Alp
A1  - Grey, Martin
A1  - Brendel, Martin
T1  - Genetic engineering of baker’s and wine yeasts using formaldehyde hyperresistance-mediating plasmids
T2  - Brazilian Journal of Medical and Biological Research
N2  - Yeast multi-copy vectors carrying the for maldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker’s and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting agent will be consumed and therefore its removal during down-stream processing is not necessary. Thus, this vector provides an expression system which is simple to apply and inexpensive to use. Key words: · Yeast · Transformation · Hyperresistance to formaldehyde
KW  - Yeast
KW  - Transformation
KW  - Hyperresistance to formaldehyde
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7141
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-71021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004
N1  - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License http://creativecommons.org/licenses/by-nc/3.0/
VL  - 30
IS  - 12
SP  - 1407
EP  - 1414
ER  -