TY  - JOUR
A1  - Michalke, Bernhard
A1  - Berthele, Achim
A1  - Venkataramani, Vivek
T1  - Simultaneous quantification and speciation of trace metals in paired serum and CSF samples by size exclusion chromatography–inductively coupled plasma–dynamic reaction cell–mass spectrometry (SEC-DRC-ICP-MS)
T2  - International journal of molecular sciences
N2  - Background: Transition metals play a crucial role in brain metabolism: since they exist in different oxidation states they are involved in ROS generation, but they are also co-factors of enzymes in cellular energy metabolism or oxidative defense. Methods: Paired serum and cerebrospinal fluid (CSF) samples were analyzed for iron, zinc, copper and manganese as well as for speciation using SEC-ICP-DRC-MS. Brain extracts from Mn-exposed rats were additionally analyzed with SEC-ICP-DRC-MS. Results: The concentration patterns of transition metal size fractions were correlated between serum and CSF: Total element concentrations were significantly lower in CSF. Fe-ferritin was decreased in CSF whereas a LMW Fe fraction was relatively increased. The 400–600 kDa Zn fraction and the Cu-ceruloplasmin fraction were decreased in CSF, by contrast the 40–80 kDa fraction, containing Cu- and Zn-albumin, relatively increased. For manganese, the α-2-macroglobulin fraction showed significantly lower concentration in CSF, whereas the citrate Mn fraction was enriched. Results from the rat brain extracts supported the findings from human paired serum and CSF samples. Conclusions: Transition metals are strictly controlled at neural barriers (NB) of neurologic healthy patients. High molecular weight species are down-concentrated along NB, however, the Mn-citrate fraction seems to be less controlled, which may be problematic under environmental load.
KW  - iron
KW  - manganese
KW  - element speciation
KW  - paired samples
KW  - cerebrospinal fluid
KW  - serum
KW  - SEC-ICP-DRC-MS
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62194
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-621948
SN  - 1422-0067
N1  - The work of B.M. and V.V. was financially supported by the Deutsche Forschungsgemeinschaft (DFG) through the Priority Program “Ferroptosis: from Molecular Basics to Clinical Applications” (SPP 2306), VE 1249/1-1.
VL  - 22.2021
IS  - 16, art. 8892
SP  - 1
EP  - 14
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -