TY  - JOUR
A1  - Jonker, Hendrik R. A.
A1  - Saxena, Krishna
A1  - Shcherbakova, Aleksandra
A1  - Tiemann, Birgit
A1  - Bakker, Hans
A1  - Schwalbe, Harald
T1  - NMR spectroscopic characterization of the C‐mannose conformation in a thrombospondin repeat using a selective labeling approach
T2  - Angewandte Chemie. International edition
N2  - Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled with NMR‐active nuclei. We report on the incorporation of 13C‐labeled mannose in the C‐mannosylated UNC‐5 thrombospondin repeat. The conformational landscape of the C‐mannose sugar puckers attached to tryptophan residues of UNC‐5 is characterized by interconversion between the canonical 1C4 state and the B03 / 1S3 state. This flexibility may be essential for protein folding and stabilization. We foresee that this versatile tool to produce proteins with selectively labeled C‐mannose can be applied and adjusted to other systems and modifications and potentially paves a way to advance glycoprotein research by unravelling the dynamical and conformational properties of glycan structures and their interactions.
KW  - C-mannosylation
KW  - conformation analysis
KW  - NMR spectroscopy
KW  - selective isotopic labeling
KW  - glycoproteins
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57019
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-570190
SN  - 1521-3773
SN  - 1433-7851
VL  - 59
IS  - 46
SP  - 20659
EP  - 20665
PB  - Wiley-VCH
CY  - Weinheim
ER  -