TY  - JOUR
A1  - Reimer, Günter
A1  - Drahovsky, Dusan
T1  - Chromosomale Strukturen von Pseudomonas testosteroni. I. Isolierung und Charakterisierung der chromosomalen Komplexe
T1  - Chromosomal structures of pseudomonas testosteroni. I. Isolation and characterization of the chromosomal complexes
T2  - Zeitschrift für Naturforschung, C
N2  - After lysis of Pseudomonas testosteroni with lysozyme and non-ionic detergents different DNA-protein complexes can be separated in 5 -25% (w/v) neutral sucrose gradient. The protein to DNA ratio of these complexes varies between 0.5-4.5 to 1, whereby the faster sedimenting forms contain more protein than the slower sedimenting ones. Different initial rates of DNase digestion may indicate various degrees of DNA packing in these complexes. The chromosomal complexes of Pseudomonas testosteroni are relatively stable towards pronase. Treatment with RNase or sodium dodecylsulphate is accompanied by a dramatic increase in viscosity and decrease in relative density. It suggests that DNA in these complexes is maintained in its supercoiled form by RNA molecule (s) in a similar way as in isolated chromosome of E. coli.
KW  - Bacterial Chromosome
KW  - Different Forms
KW  - Deoxyribonuclease Digestion
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72104
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-721049
SN  - 0939-5075
SN  - 1865-7125
VL  - 31.1976
IS  - 1-2
SP  - 91
EP  - 97
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -