TY  - JOUR
A1  - Kahl, Günter
A1  - Lange, Horst
A1  - Rosenstock, Günter
T1  - Substratspiegel, Enzymaktivitäten und genetische Regulation nach Derepression in pflanzlichen Speichergeweben
T1  - Substrate levels, enzymatic activities and genetic regulation after derepression in plant storage tissues
T2  - Zeitschrift für Naturforschung, B
N2  - Differential derepression of the genome of potato tuber cells can be initiated by slicing the tissue into disks. The consequence of this procedure on the cells of the wound surface is dedifferentiation and cell division followed by redifferentiation to a suberized phellem cell. The drift of glucose-, glucose-1-phosphate-, glucose-6-phosphate-, fructose-6-phosphate- and 6-phospho-gluconatelevels has been determined in the derepressed tissue. With the exception of 6-phospho-gluconate all intermediates so far investigated showed a rise in concentration after derepression.
This is interpreted as a consequence of altered enzymic activities which were estimated for phosphoglucomutase, hexokinase, phosphoglucoisomerase, gluco-6-phosphate- and 6-phosphogluconatedehydrogenase. The two dehydrogenases were activated after derepression, the activation represented a de-novo-synthesis, as was demonstrated with the inhibitors Actidione (translation) and p-Fluorophenyl-alanine (protein synthesis in general). Hexokinase and phosphoglucoisomerase were not severely affected by cutting the tissue. Phosphoglucomutase was degrated rapidly, the degradation being dependent on protein synthesis. The importance of an enhanced activity of the pentose phosphate shunt for the stressed cell is emphasized and the possibility of an alteration in the osmotic pressure within the cell and especially in the nucleus — a primary consequence of wounding — as a cause of derepression in potato tuber cells is discussed.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74899
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-748998
SN  - 1865-7117
SN  - 0932-0776
VL  - 24
IS  - 7
SP  - 911
EP  - 918
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -