TY  - JOUR
A1  - Namgaladze, Dmitry
A1  - Jennewein, Carla
A1  - Preiss, Stefan
A1  - Knethen, Andreas von
A1  - Brüne, Bernhard
T1  - Attenuated suppression of the oxidative burst by cells dying in the presence of oxidized low density lipoprotein
T2  - Journal of lipid research
N2  - Macrophages ingesting apoptotic cells attenuate inflammatory responses, such as reactive oxygen species (ROS) generation. In atherosclerosis, ongoing inflammation and accumulation of apoptotic/necrotic material are observed, suggesting defects of phagocytes in recognizing or responding to dying cells. Modified lipoproteins such as oxidized LDL (oxLDL) are known to promote inflammation and to interfere with apoptotic cell clearance. Here, we studied the impact of cells exposed to oxLDL on their ability to interfere with the oxidative burst in phagocytes. In contrast to apoptotic cells, cells dying in response to or in the presence of oxLDL failed to suppress ROS generation despite efficiently being taken up by phagocytes. In addition, apoptotic cells, but not oxLDL-treated cells, inhibited phosphorylation of extracellular signal-regulated kinase, which is important for NADPH oxidase activation. oxLDL treatment did not interfere with activation of the antiinflammatory transcriptional regulator peroxisome proliferator-activated receptor gamma by apoptotic cells. Moreover, cells exposed to oxLDL failed to suppress lipopolysaccharide- induced proinflammatory cytokine expression, whereas apoptotic cells attenuated these phagocyte responses. Thus, the presence of oxLDL during cell death impaired the ability of apoptotic cells to act antiinflammatory with regard to oxidative burst inhibition and cytokine expression in phagocytes.
KW  - apoptosis
KW  - macrophages
KW  - NADPH oxidase
KW  - lipoproteins
KW  - phagocytosis
KW  - reactive oxygen species
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76454
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-764545
SN  - 0022-2275
VL  - 50.2009
IS  - 11
SP  - 2173
EP  - 2181
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -