TY  - JOUR
A1  - Bausewein, Thomas
A1  - Nußberger, Stephan
A1  - Kühlbrandt, Werner
T1  - Cryo-EM structure of Neurospora crassa respiratory complex IV
T2  - IUCrJ
N2  - In fungi, the mitochondrial respiratory chain complexes (complexes I–IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from Neurospora crassa in lipid nanodiscs as cytochrome c oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from Saccharomyces cerevisiae. Its ten subunits are conserved in S. cerevisiae and Bos taurus, but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to N. crassa.
KW  - 3D reconstruction and image processing
KW  - automation
KW  - single-particle cryo-EM
KW  - structure determination
KW  - cryo-electron microscopy
KW  - cytochrome c oxidase
KW  - Neurospora crassa
KW  - respiratory complex IV
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75801
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-758018
SN  - 2052-2525
VL  - 6
IS  - 4
SP  - 773
EP  - 780
PB  - International Union of Crystallography
CY  - Chester
ER  -