TY  - JOUR
A1  - Youhanna, Sonia
A1  - Lauschke, Volker Martin
T1  - The past, present and future of intestinal in vitro cell systems for drug absorption studies
T2  - Journal of Pharmaceutical Sciences
N2  - The intestinal epithelium acts as a selective barrier for the absorption of water, nutrients and orally administered drugs. To evaluate the gastrointestinal permeability of a candidate molecule, scientists and drug developers have a multitude of cell culture models at their disposal. Static transwell cultures constitute the most extensively characterized intestinal in vitro system and can accurately categorize molecules into low, intermediate and high permeability compounds. However, they lack key aspects of intestinal physiology, including the cellular complexity of the intestinal epithelium, flow, mechanical strain, or interactions with intestinal mucus and microbes. To emulate these features, a variety of different culture paradigms, including microfluidic chips, organoids and intestinal slice cultures have been developed. Here, we provide an updated overview of intestinal in vitro cell culture systems and critically review their suitability for drug absorption studies. The available data show that these advanced culture models offer impressive possibilities for emulating intestinal complexity. However, there is a paucity of systematic absorption studies and benchmarking data and it remains unclear whether the increase in model complexity and costs translates into improved drug permeability predictions. In the absence of such data, conventional static transwell cultures remain the current gold-standard paradigm for drug absorption studies.
KW  - Drug permeability
KW  - Bioavailability prediction
KW  - Microfluidics
KW  - Organoids
KW  - Microphysiological models
KW  - Preclinical drug development
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62386
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-623863
SN  - 1520-6017
VL  - 110.2021
IS  - 1
SP  - 50
EP  - 65
PB  - Elsevier
CY  - Amsterdam
ER  -