TY  - JOUR
A1  - Strobl, Frederic
A1  - Stelzer, Ernst H. K.
T1  - A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
T2  - Scientific reports
N2  - We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
KW  - Entomology
KW  - Genetic vectors
KW  - Genotyping and haplotyping
KW  - Transgenic organisms
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62779
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-627795
SN  - 2045-2322
N1  - Open Access funding enabled and organized by Projekt DEAL. The research was funded by the Cluster of Excellence Frankfurt for Macromolecular Complexes (CEF-MC, EXC 115) granted to the Buchmann Institute for Molecular Life Sciences (BMLS) at the Goethe-Universität Frankfurt am Main by the Deutsche Forschungsgemeinschaft (DFG) and Joachim Herz Stiftung, Addon Fellowship 2019.
VL  - 11
IS  - art. 15325
SP  - 1
EP  - 9
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - [London]
ER  -