TY  - JOUR
A1  - Aufurth, Sascha
A1  - Schägger, Hermann
A1  - Müller, Volker
T1  - Identification of subunits a, b, and c1 from Acetobacterium woodii Na+-F1F0-ATPase : subunits c1, c2, AND c3 constitute a mixed c-oligomer
T2  - Journal of biological chemistry
N2  - The Na+-F1F0-ATPase operon ofAcetobacterium woodii was recently shown to contain, among eleven atp genes, those genes that encode subunita and b, a gene encoding a 16-kDa proteolipid (subunit c 1), and two genes encoding 8-kDa proteolipids (subunits c 2 andc 3). Because subunits a,b, and c 1 were not found in previous enzyme preparations, we re-determined the subunit composition of the enzyme. The genes were overproduced, and specific antibodies were raised. Western blots revealed that subunits a,b, and c 1 are produced and localized in the cytoplasmic membrane. Membrane protein complexes were solubilized by dodecylmaltoside and separated by blue native-polyacrylamide gel electrophoresis, and the ATPase subunits were resolved by SDS-polyacrylamide gel electrophoresis. N-terminal sequence analyses revealed the presence of subunitsa, c 2, c 3,b, δ, α, γ, β, and ε. Biochemical and immunological analyses revealed that subunitsc 1, c 2, andc 3 are all part of the c-oligomer, the first of a F1F0-ATPase that contains 8- and 16-kDa proteolipids.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75837
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-758373
SN  - 0021-9258
VL  - 275.2000
IS  - 43
SP  - 33297
EP  - 33301
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -