TY  - JOUR
A1  - Zimmer, Guido
A1  - Günther, Herbert O.
A1  - Schmidt, Hartmut
T1  - Interaction of phloretin with the human red cell membrane and membrane lipids: evidence from infrared, Raman and ESR spectroscopy
T2  - Zeitschrift für Naturforschung, C
N2  - Membrane-Phloretin Interaction, Infrared Raman, ESR Spectroscopy The transport inhibitor phloretin was bound to human red cell membrane and the concomitant structural changes were observed by spectroscopic methods. By the spin labeling method a decrease in fluidity of the membrane was found at 1 and 10 |iM concentrations of the reagent. This result was obtained with the 2-(3-Carboxypropyl)-4,4-dimethyl-2-tridecyl-3-oxazolidinyloxyl, and the 2-(14-Carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl lipid spin labels. Infrared spectroscopy of modified membranes revealed an intensity increase of the POO~ band at about 1250 cm-1. Moreover, a shift of the peak at 1050 cm -1 to 1100 cm-1 was observed in the presence of phloretin. Raman spectroscopy of the membranes did not contradict the results found with infrared and ESR spectroscopy: In the phloretin modified membrane we observed a lack of the band at 1085 cm-1, which leads to suggest that the POO" and/or C-C regions are less fluid. Changes of the extracted red cell membrane lipids were less characteristic, and the results differed from those found in red cell membrane.
KW  - Membrane-Phloretin Interaction
KW  - Infrared Raman
KW  - ESR Spectroscopy
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/80638
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-806386
SN  - 0939-5075
SN  - 1865-7125
VL  - 36.1981
IS  - 7-8
SP  - 586
EP  - 592
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -