TY  - JOUR
A1  - Rajalingam, Krishnaraj
A1  - Sharma, Manu
A1  - Paland, Nicole
A1  - Hurwitz, Robert
A1  - Thieck, Oliver
A1  - Oswald, Monique
A1  - Machuy, Nikolaus
A1  - Rudel, Thomas
T1  - IAP-IAP complexes required for apoptosis resistance of C. trachomatis-infected cells
T2  - PLoS pathogens
N2  - Host cells infected with obligate intracellular bacteria Chlamydia trachomatis are profoundly resistant to diverse apoptotic stimuli. The molecular mechanisms underlying the block in apoptotic signaling of infected cells is not well understood. Here we investigated the molecular mechanism by which apoptosis induced via the tumor necrosis factor (TNF) receptor is prevented in infected epithelial cells. Infection with C. trachomatis leads to the up-regulation of cellular inhibitor of apoptosis (cIAP)-2, and interfering with cIAP-2 up-regulation sensitized infected cells for TNF-induced apoptosis. Interestingly, besides cIAP-2, cIAP-1 and X-linked IAP, although not differentially regulated by infection, are required to maintain apoptosis resistance in infected cells. We detected that IAPs are constitutively organized in heteromeric complexes and small interfering RNA-mediated silencing of one of these IAPs affects the stability of another IAP. In particular, the stability of cIAP-2 is modulated by the presence of X-linked IAP and their interaction is stabilized in infected cells. Our observations suggest that IAPs are functional and stable as heteromers, a thus far undiscovered mechanism of IAP regulation and its role in modulation of apoptosis.
Y1  - 2006
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/27239
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-272392
SN  - 1553-7374
SN  - 1553-7366
N1  - Copyright: © 2006 Rajalingam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 2
IS  - (10): e114
SP  - 1013
EP  - 1023
PB  - PLoS
CY  - Lawrence, Kan.
ER  -