TY  - JOUR
A1  - Wacker, Adolf
A1  - Ishimoto, Makoto
A1  - Chandra, Prakash
A1  - Selzer, Reinhold
T1  - Photoreaktivierung von UV-inaktivierter Polyuridylsäure
T2  - Zeitschrift für Naturforschung, B
N2  - A study on the effect of UV-irradiated polyuridylic acid on the incorporation of phenylalanine into the polypeptide precipitable through trichloroacetic acid, in a cell-free system from E. coli was made. Attempts were made to reactivate the UV-inactivated polyuridylic acid through hydrogen peroxide, uranyl acetate and visible light. We could show that polyuridylic acid irradiated at a dose of 1.2 ×105 ergs/mm2 could be completely reactivated, while the one irradiated at a higher dose of 2.4 ×105 ergs/mm2 could not be completely reactivated under the conditions of our experiment. We have studied the effects of hydrogen peroxide and uranyl acetate on UV-irradiated polyuridylic acid chemically as well. Our results altogether show that the photoreactivating effect of uranyl acetate and hydrogen peroxide is due to their ability to split the uracil dimers formed during UV-irradiation.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/71317
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-713173
SN  - 1865-7117
SN  - 0932-0776
VL  - 19
IS  - 5
SP  - 406
EP  - 408
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -