TY  - JOUR
A1  - Eschemann, Andrea
A1  - Galkin, Alexander
A1  - Oettmeier, Walter
A1  - Brandt, Ulrich
A1  - Kerscher, Stefan
T1  - HDQ (1-hydroxy-2-dodecyl-4(1H)quinolone), a high affinity inhibitor for mitochondrial alternative NADH dehydrogenase: evidence for a ping-pong mechanism
T2  - Journal of biological chemistry
N2  - Alternative NADH dehydrogenases (NADH:ubiquinone oxidoreductases) are single subunit respiratory chain enzymes found in plant and fungal mitochondria and in many bacteria. It is unclear how these peripheral membrane proteins interact with their hydrophobic substrate ubiquinone. Known inhibitors of alternative NADH dehydrogenases bind with rather low affinities. We have identified 1-hydroxy-2-dodecyl-4(1H)quinolone as a high affinity inhibitor of alternative NADH dehydrogenase from Yarrowia lipolytica. Using this compound, we have analyzed the bisubstrate and inhibition kinetics for NADH and decylubiquinone. We found that the kinetics of alternative NADH dehydrogenase follow a ping-pong mechanism. This suggests that NADH and the ubiquinone headgroup interact with the same binding pocket in an alternating fashion.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75905
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-759055
SN  - 0021-9258
VL  - 280
IS  - 5
SP  - 3138
EP  - 3142
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -