TY  - JOUR
A1  - Giorno, Filomena
A1  - Wolters-Arts, Mieke
A1  - Grillo, Stefania
A1  - Scharf, Klaus-Dieter
A1  - Vriezen, Wim H.
A1  - Mariani, Celestina
T1  - Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers
T2  - The journal of experimental botany
N2  - The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures. Key words: Anther development, heat stress, HsfA2, Hsp17-CII, pollen, tomato
KW  - anther development
KW  - heat stress
KW  - HsfA2
KW  - Hsp17-CII
KW  - pollen
KW  - tomato
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7359
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-73387
SN  - 1460-2431
SN  - 0022-0957
N1  - © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
VL  - 61
IS  - 2
SP  - 453
EP  - 462
PB  - Oxford Univ. Press
CY  - Oxford
ER  -