TY  - JOUR
A1  - Polyakova, Maryna
A1  - Schlögl, Haiko
A1  - Sacher, Julia
A1  - Schmidt-Kassow, Maren
A1  - Kaiser, Jochen
A1  - Stumvoll, Michael
A1  - Kratzsch, Jürgen
A1  - Schroeter, Matthias
T1  - Stability of BDNF in human samples stored up to 6 months and correlations of serum and EDTA-plasma concentrations
T2  - International journal of molecular sciences
N2  - Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.
KW  - BDNF
KW  - brain-derived neurotrophic factor
KW  - serum
KW  - EDTA-plasma
KW  - stability
KW  - inter-assay precision
KW  - acceptable change limit
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45070
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-450707
SN  - 1422-0067
SN  - 1661-6596
N1  - © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 18
IS  - 6, Art. 1189
SP  - 1
EP  - 11
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -