TY  - JOUR
A1  - Palm, Denise
A1  - Streit, Deniz
A1  - Ruprecht, Maike
A1  - Simm, Stefan
A1  - Scharf, Christian
A1  - Schleiff, Enrico
T1  - Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae
T2  - FEBS Open Bio
N2  - Ribosome biogenesis is essential for cellular function and involves rRNA synthesis, rRNA processing and modification, and ribosomal protein assembly. Ribosome biogenesis factors and small nucleolar RNA assist these events. Ribosomal maturation takes place in the nucleolus, the nucleoplasm, and the cytosol in a coordinated and controlled manner. For example, some ribosomal proteins are thought to be assembled in the cytoplasm based on the observations in Saccharomyces cerevisiae. Here, we used cellular fractionation to demonstrate that cleavage of the 20S intermediate, the precursor to mature 18S rRNA, does not occur in the nucleoplasm of Arabidopsis thaliana. It most likely occurs in the cytoplasm. Further, we verified the proposed localization of RPS10e, RPS26e, and RPL24a/b in the nucleus and RPP1 in the nucleolus of A. thaliana by ribosome profiling, immunofluorescence, and analysis of the localization of GFP fusion proteins. Our results suggest that the order of events during ribosomal protein assembly in the ribosome biogenesis pathway differs between plants and yeast.
KW  - Arabidopsis thaliana
KW  - eukaryotic system
KW  - intracellular protein localization
KW  - ribosomal proteins
KW  - ribosome biogenesis
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46937
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-469372
SN  - 2211-5463
N1  - © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
VL  - 8
IS  - 9
SP  - 1437
EP  - 1444
PB  - Elsevier on behalf of the Federation of European Biochemical Societies
CY  - Cambridge
ER  -