TY  - JOUR
A1  - Acuña-Hinrichsen, Francisca
A1  - Covarrubias-Pinto, Adriana
A1  - Ishizuka, Yuta
A1  - Stolzenbach, María Francisca
A1  - Martin, Carolina
A1  - Salazar, Paula
A1  - Castro, Maite A.
A1  - Bramham, Clive R.
A1  - Otth, Carola
T1  - Herpes simplex virus type 1 neuronal infection triggers the disassembly of key structural components of dendritic spines
T2  - Frontiers in cellular neuroscience
N2  - Herpes simplex virus type 1 (HSV-1) is a widespread neurotropic virus. Primary infection of HSV-1 in facial epithelium leads to retrograde axonal transport to the central nervous system (CNS) where it establishes latency. Under stressful conditions, the virus reactivates, and new progeny are transported anterogradely to the primary site of infection. During the late stages of neuronal infection, axonal damage can occur, however, the impact of HSV-1 infection on the morphology and functional integrity of neuronal dendrites during the early stages of infection is unknown. We previously demonstrated that acute HSV-1 infection in neuronal cell lines selectively enhances Arc protein expression - a major regulator of long-term synaptic plasticity and memory consolidation, known for being a protein-interaction hub in the postsynaptic dendritic compartment. Thus, HSV-1 induced Arc expression may alter the functionality of infected neurons and negatively impact dendritic spine dynamics. In this study we demonstrated that HSV-1 infection induces structural disassembly and functional deregulation in cultured cortical neurons, an altered glutamate response, Arc accumulation within the somata, and decreased expression of spine scaffolding-like proteins such as PSD-95, Drebrin and CaMKIIβ. However, whether these alterations are specific to the HSV-1 infection mechanism or reflect a secondary neurodegenerative process remains to be determined.
KW  - Herpes Simplex Virus Type 1 (HSV-1)
KW  - neurodegeneration
KW  - neurotropic virus
KW  - Arc protein
KW  - memory consolidation
KW  - dendritic spines
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62019
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-620197
SN  - 1662-5102
N1  - These studies were funded by the following grants: Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) REGULAR 1180936 (CO), FONDECYT REGULAR 1150574 (CO), FONDECYT REGULAR 1151206 and 1191620 (MAC), The Research Council of Norway, Toppforsk grant/249951 (CRB) and CONICYT 21150756 (FA-H), CISNe-UACh, and DID-UACh.
VL  - 15
IS  - art. 580717
SP  - 1
EP  - 18
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  -