TY  - JOUR
A1  - Hildebrand, Sven
A1  - Schueth, Anna
A1  - Wangenheim, Klaus von
A1  - Mattheyer, Christian
A1  - Pampaloni, Francesco
A1  - Bratzke, Hansjürgen
A1  - Roebroeck, Alard F.
A1  - Galuske, Ralf
T1  - hFRUIT: an optimized agent for optical clearing of DiI-stained adult human brain tissue
T2  - Scientific reports
N2  - Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54772
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-547728
SN  - 2045-2322
IS  - Article number 9950
PB  - Nature Publishing Group
ER  -