TY  - JOUR
A1  - Grebenovsky, Nikolai Michal
A1  - Luma, Larita
A1  - Müller, Patricia
A1  - Heckel, Alexander
T1  - Introducing LNAzo: more rigidity for improved photocontrol of oligonucleotide hybridization
T2  - Chemistry – a European journal
N2  - Oligonucleotide-based therapeutics have made rapid progress in clinical treatment of a variety of disease indications. Since most therapeutic oligonucleotides serve more than just one function and tend to have a prolonged lifetime, spatio-temporal control of these functions would be desirable. Photoswitches like azobenzene have proven themselves as useful tools in this matter. Upon irradiation, the photoisomerization of the azobenzene moiety causes destabilization in adjacent base pairs, leading to a decreased hybridization affinity. Since the way the azobenzene is incorporated in the oligonucleotide is of utmost importance, we synthesized locked azobenzene C-nucleosides and compared their photocontrol capabilities to established azobenzene C-nucleosides in oligonucleotide test-sequences by means of fluorescence-, UV/Vis-, and CD-spectroscopy.
KW  - azo compounds
KW  - DNA
KW  - oligonucleotides
KW  - photochemistry
KW  - RNA
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79213
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-792134
SN  - 1521-3765
N1  - This work was supported by the Deutsche Forschungsgesellschaft through SFB902 (Molecular Principles of RNA-based Regulation). Open access funding enabled and organized by Projekt DEAL.
VL  - 25
IS  - 53
SP  - 12298
EP  - 12302
PB  - Wiley-VCH
CY  - Weinheim
ER  -