TY  - JOUR
A1  - De Vargas Roditi, Laura
A1  - Jacobs, Andrea
A1  - Rueschoff, Jan Hendrik
A1  - Bankhead, Pete
A1  - Chevrier, Stéphane
A1  - Jackson, Hartland W.
A1  - Hermanns, Thomas
A1  - Fankhauser, Christian D.
A1  - Poyet, Cedric
A1  - Chun, Felix
A1  - Rupp, Niels Jan Felix
A1  - Tschäbunin, Alexandra
A1  - Bodenmiller, Bernd
A1  - Wild, Peter Johannes
T1  - Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer
T2  - Cell reports medicine
N2  - Localized prostate cancer exhibits multiple genomic alterations and heterogeneity at the proteomic level. Single-cell technologies capture important cell-to-cell variability responsible for heterogeneity in biomarker expression that may be overlooked when molecular alterations are based on bulk tissue samples. This study aims to identify prognostic biomarkers and describe the heterogeneity of prostate cancer and the associated microenvironment by simultaneously quantifying 36 proteins using single-cell mass cytometry analysis of over 1.6 million cells from 58 men with localized prostate cancer. We perform this task, using a high-dimensional clustering pipeline named Franken to describe subpopulations of immune, stromal, and prostate cells, including changes occurring in tumor tissues and high-grade disease that provide insights into the coordinated progression of prostate cancer. Our results further indicate that men with localized disease already harbor rare subpopulations that typically occur in castration-resistant and metastatic disease.
KW  - single-cell proteomics
KW  - prostate cancer
KW  - cellular heterogeneity
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78264
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-782643
SN  - 2666-3791
VL  - 3
IS  - 4, 100604
PB  - Elsevier
CY  - Amsterdam
ER  -